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The presence of cotinine in the saliva, urine, and blood of non-smokers, even in its smallest quantity, is evident in secondhand smoking

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Until recently, genetic testing was principally based on (serial) single-gene testing by Sanger sequencing, i.e., analysis of COL5A1 is performed first, followed by COL5A2 analysis and then gene-targeted copy number variant analysis to identify large deletions or duplications

Furthermore, cotinine may be present in the body for up to four days if smoking is infrequent